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Aurora A Antikörper (C-Term)

Dieses Anti-Aurora A-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Aurora A in WB, ELISA und IP. Geeignet für Human.
Produktnummer ABIN184572

Kurzübersicht für Aurora A Antikörper (C-Term) (ABIN184572)

Target

Alle Aurora A (AURKA) Antikörper anzeigen
Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität

  • 119
  • 69
  • 42
  • 21
  • 18
  • 16
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
Human

Wirt

  • 115
  • 20
Ziege

Klonalität

  • 104
  • 30
Polyklonal

Konjugat

  • 86
  • 8
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Aurora A Antikörper ist unkonjugiert

Applikation

  • 100
  • 54
  • 39
  • 30
  • 16
  • 14
  • 11
  • 10
  • 10
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Güteklasse

Verified
  • Bindungsspezifität

    • 22
    • 11
    • 8
    • 8
    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Verwendungszweck

    Aurora kinase A / AURKA

    Sequenz

    QNKESASKQS

    Spezifität

    Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

    Kreuzreaktivität

    Human

    Aufreinigung

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

    Isotyp

    IgG
  • Applikationshinweise

    Western Blot: Approx 45 kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8 kDa according to NP_003591.2). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Kommentare

    Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Minimize freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    Aurora A (AURKA) (Aurora Kinase A (AURKA))

    Andere Bezeichnung

    AURKA

    Hintergrund

    AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser

    Gen-ID

    6790

    NCBI Accession

    NP_003591

    Pathways

    Zellzyklus, Asymmetric Protein Localization
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