DYKDDDDK Tag Antikörper (iFluor 555)

Produktnummer ABIN1842288

Der iFluor 555-konjugierte Maus Monoklonal anti-DYKDDDDK Tag Antikörper (ABIN1842288) detektiert spezifisch DYKDDDDK Tag.

Kurzübersicht für DYKDDDDK Tag Antikörper (iFluor 555) (ABIN1842288)

Target: DYKDDDDK Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist konjugiert mit iFluor 555

Applikation: Bitte anfragen

Produktdetails anti-DYKDDDDK Tag Antikörper (iFluor 555)

Sequenz: DYKDDDDK

Spezifität: THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide DYKDDDDK coupled to KLH

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Konzentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Substanzklasse: Tag

Hintergrund: The DYKDDDK tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-DYKDDDK tag antibody is a useful tool for the analysis of Flag-tagged proteins.THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.