DLL4 Antikörper

Produktnummer ABIN6796404

Dieses Anti-DLL4-Antikörper ist ein Hamster Monoklonal-Antikörper zur Detektion von DLL4 in IHC, FACS, IHC (p), Func und IHC (fro). Geeignet für Maus.

Kurzübersicht für DLL4 Antikörper (ABIN6796404)

Target: DLL4 (delta-Like 4 (DLL4))

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser DLL4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: HMD4-2

Produktdetails anti-DLL4 Antikörper

Spezifität: Recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non-productive angiogenesis. Clone HMD4-2 has been shown to block mouse Notch1-Fc and Notch4-Fc binding to mouse DLL4/CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205. Removal of Sodium Azide is recommended prior to use in functional assays.

Aufreinigung: Protein G purified

Immunogen: Recombinant mouse DLL4.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Andere Bezeichnung: DLL4

Hintergrund: Name/Gene ID: DLL4

Synonyms: DLL4, Delta-like 4 protein, Delta like 4, Delta-like 4 (Drosophila), Delta-like 4 homolog, Drosophila Delta homolog 4, Notch ligand DLL4, Delta 4, Delta ligand 4, Delta-like protein 4, Delta4, Hdelta2, Notch ligand delta-2

Gen-ID: 54567

UniProt: Q9NR61

Pathways: Notch Signalweg