CTGF Antikörper

Produktnummer ABIN181700

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CTGF in WB, IHC (p), EIA und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CTGF Antikörper (ABIN181700)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CTGF Antikörper

Spezifität: This antibody detects Human Connective Tissue Growth Factor (CTGF). Other species not tested.

Aufreinigung: Immunoaffinity Chromatography employing immobilized Human CTGF matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human CTGF.

Anwendungsinformationen

Applikationshinweise: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman CTGF (5.0 μg/mL) a concentration of 6.0-9.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Human CTGF (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This affinity purified antibody,in conjunction with Biotinylated antibody Human CTGF (AP01150BT) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF. Western blot: To detect Human CTGF this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Andere Bezeichnung: CTGF

Hintergrund: CTGF (connective tissue growth factor) is a 38 kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.Synonyms: CCN family member 2, CCN2, Connective tissue growth factor, HCS24, Hypertrophic chondrocyte-specific protein 24, IBP8, IGF-binding protein 8, IGFBP8, Insulin-like growth factor-binding protein 8

Gen-ID: 1490

NCBI Accession: NP_001892

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding