CTGF Antikörper

Produktnummer ABIN181700

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CTGF in WB, IHC (p), EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CTGF Antikörper (ABIN181700)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CTGF Antikörper

Spezifität: This antibody detects Human Connective Tissue Growth Factor (CTGF). Other species not tested.

Aufreinigung: Immunoaffinity Chromatography employing immobilized Human CTGF matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human CTGF.

Anwendungsinformationen

Applikationshinweise: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman CTGF (5.0 μg/mL) a concentration of 6.0-9.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Human CTGF (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This affinity purified antibody,in conjunction with Biotinylated antibody Human CTGF (AP01150BT) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF. Western blot: To detect Human CTGF this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Andere Bezeichnung: CTGF

Hintergrund: CTGF (connective tissue growth factor) is a 38 kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.Synonyms: CCN family member 2, CCN2, Connective tissue growth factor, HCS24, Hypertrophic chondrocyte-specific protein 24, IBP8, IGF-binding protein 8, IGFBP8, Insulin-like growth factor-binding protein 8

Gen-ID: 1490

NCBI Accession: NP_001892

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding