CXCL14 Antikörper

Produktnummer ABIN181664

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CXCL14 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CXCL14 Antikörper (ABIN181664)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CXCL14 Antikörper

Spezifität: This antibody detects BRAK.

Aufreinigung: Immunoaffinity Chromatography using an immobilized Human BRAK matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human BRAK

Anwendungsinformationen

Applikationshinweise: ELISA: Indirect: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK. Sandwich: To detect hBRAK by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human BRAK as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hBRAK. Western blot: To detect hBRAK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Andere Bezeichnung: CXCL14 / BRAK

Hintergrund: BRAK/CXCL14 is a CXC chemokine constitutively expressed at the mRNA level in certain normal tissues but absent from many established tumor cell lines and human cancers. Although multiple investigators have cloned BRAK, little is known regarding the physiologic function of BRAK or the reason for decreased expression in cancer.Synonyms: C-X-C motif chemokine 14, NJAC, SCYB14, Small-inducible cytokine B14

Gen-ID: 9547

NCBI Accession: NP_004878

UniProt: O95715

Pathways: Autophagie