CXCL14 Antikörper (Biotin)

Produktnummer ABIN181661

Der Kaninchen Polyklonal Anti-CXCL14-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CXCL14 in Proben von Human zu detektieren.

Kurzübersicht für CXCL14 Antikörper (Biotin) (ABIN181661)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL14 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CXCL14 Antikörper (Biotin)

Spezifität: This antibody detects BRAK.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BRAK

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK. Sandwich: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. This In conjunction with PolyclonalAnti-Human BRAK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/wellof recombinant hBRAK. Western blot: To detect hBRAK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restoree in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Andere Bezeichnung: CXCL14 / BRAK

Hintergrund: BRAK/CXCL14 is a CXC chemokine constitutively expressed at the mRNA level in certain normal tissues but absent from many established tumor cell lines and human cancers. Although multiple investigators have cloned BRAK, little is known regarding the physiologic function of BRAK or the reason for decreased expression in cancer.Synonyms: C-X-C motif chemokine 14, NJAC, SCYB14, Small-inducible cytokine B14

Gen-ID: 9547

NCBI Accession: NP_004878

UniProt: O95715

Pathways: Autophagie