IGFBP5 Antikörper

Produktnummer ABIN181656

Der Kaninchen Polyklonal Anti-IGFBP5-Antikörper wurde für WB und EIA validiert. Er ist geeignet, IGFBP5 in Proben von Human zu detektieren.

Kurzübersicht für IGFBP5 Antikörper (ABIN181656)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGFBP5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IGFBP5 Antikörper

Spezifität: This antibody detects Insulin-like Growth Factor Binding Protein 5.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98 %) recombinant human IGF-BP5

Anwendungsinformationen

Applikationshinweise: Indirect ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Sandwich ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIGF-BP5 (ABIN181653 or ABIN181654) as a detection antibody, it allows the detection ofat least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Andere Bezeichnung: IGFBP5

Hintergrund: IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Synonyms: IBP-5, IBP5, IGF-binding protein 5, IGFBP-5, Insulin-like growth factor-binding protein 5

Gen-ID: 3488

NCBI Accession: NP_000590

UniProt: P24593

Pathways: WNT Signalweg, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding