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CD40 Ligand Antikörper (Biotin)

CD40LG Reaktivität: Human WB, EIA Wirt: Ziege Polyclonal Biotin
Produktnummer ABIN181578
  • Target Alle CD40 Ligand (CD40LG) Antikörper anzeigen
    CD40 Ligand (CD40LG)
    Reaktivität
    • 157
    • 82
    • 34
    • 9
    • 8
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 93
    • 76
    • 25
    • 12
    • 11
    • 2
    Ziege
    Klonalität
    • 127
    • 87
    • 4
    Polyklonal
    Konjugat
    • 111
    • 23
    • 21
    • 14
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD40 Ligand Antikörper ist konjugiert mit Biotin
    Applikation
    • 120
    • 101
    • 57
    • 50
    • 40
    • 29
    • 22
    • 18
    • 16
    • 13
    • 13
    • 13
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody detects Human CD154 / sCD40L.
    Aufreinigung
    Affinity Chromatography
    Immunogen
    Highly pure (> 98%) E.coli derived recombinant Human sCD40L
    Top Product
    Discover our top product CD40LG Primärantikörper
  • Applikationshinweise
    ELISA: Direct: To detect hsCD40L by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L. Sandwich: To detect hsCD40L by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumansCD40L as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hsCD40L. Western Blot: To detect hsCD40L by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the lyophilized antibody at -20 °C. Following reconstitution the antibody it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    Haltbarkeit
    6 months
  • Target
    CD40 Ligand (CD40LG)
    Andere Bezeichnung
    CD154 / CD40L (CD40LG Produkte)
    Hintergrund
    CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
    Gen-ID
    959
    NCBI Accession
    NP_000065
    UniProt
    P29965
    Pathways
    NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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