VEGFA Antikörper (Biotin)

Produktnummer ABIN181546

Der Biotin-konjugierte Ziege Polyklonal anti-VEGFA Antikörper (ABIN181546) detektiert spezifisch VEGFA in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für VEGFA Antikörper (Biotin) (ABIN181546)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-VEGFA Antikörper (Biotin)

Spezifität: This antibody detects VEGF

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human VEGF

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hVEGF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant VEGF. Western Blot: To detect VEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGF-A

Hintergrund: Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gen-ID: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling