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IGFBP7 Antikörper

Der Kaninchen Polyklonal Anti-IGFBP7-Antikörper wurde für WB und EIA validiert. Er ist geeignet, IGFBP7 in Proben von Human zu detektieren.
Produktnummer ABIN181541

Kurzübersicht für IGFBP7 Antikörper (ABIN181541)

Target

Alle IGFBP7 Antikörper anzeigen
IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reaktivität

  • 64
  • 34
  • 26
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 63
  • 9
  • 2
  • 1
  • 1
Kaninchen

Klonalität

  • 59
  • 14
  • 2
Polyklonal

Konjugat

  • 41
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser IGFBP7 Antikörper ist unkonjugiert

Applikation

  • 50
  • 23
  • 23
  • 13
  • 13
  • 13
  • 13
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Spezifität

    This antibody detects Human IGFBP7. Other species not tesed.

    Aufreinigung

    Affinity Chromatography employing immobilized Human IGF-BP7 matrix

    Immunogen

    Highly pure (> 98%) E.coli recombinant Human IGFBP7
  • Applikationshinweise

    Sandwich ELISA: To detect hIGF-BP7 (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIGF-BP7 (ABIN181539 or ABIN181540) as a detection antibody, it allows the detection ofat least 0.2-0.4 ng/well of recombinant hIGF-BP7. Western blot: To detect hIGF-BP7 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIGF-BP7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

    Haltbarkeit

    12 months
  • Target

    IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

    Andere Bezeichnung

    IGFBP7

    Hintergrund

    IGF-BPs form high affinity complexes with both IGF-I and IGF-II and act to control of the distribution, function and activity of IGFs in various cell tissues and body fluids. There are seven named IGF-BPs. IGFBP7 plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. IGFBP7 is expressed in a wide range of normal human tissues and it usually shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin.Synonyms: IBP-7, IBP7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, Insulin-like growth factor-binding protein 7, MAC25, PGI2-stimulating factor, PSF, Prostacyclin-stimulating factor, TAF, Tumor-derived adhesion factor

    Gen-ID

    3490

    NCBI Accession

    NP_001544

    UniProt

    Q16270

    Pathways

    Growth Factor Binding
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