Calreticulin Antikörper (Internal Region)

Produktnummer ABIN1782157

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Calreticulin in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für Calreticulin Antikörper (Internal Region) (ABIN1782157)

Target: Calreticulin (CALR)

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Calreticulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-Calreticulin Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: calreticulin

Sequenz: HPEIDNPEYS PDPS

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1 kDa according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14,270(28)

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Calreticulin

Target: Calreticulin (CALR)

Andere Bezeichnung: CALR

Hintergrund: CALR, calreticulin, CRT, RO, SSA, cC1qR, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro, calreticulin), calregulin, endoplasmic reticulum resident protein 60, grp60

Molekulargewicht: Expected molecular weight 60 kDa

Gen-ID: 811, 12317, 64202

NCBI Accession: NP_004334

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response