GRIA2 Antikörper (AA 278-292)

Produktnummer ABIN1742378

Der Maus Monoklonal Anti-GRIA2-Antikörper wurde für WB und IP validiert. Er ist geeignet, GRIA2 in Proben von Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GRIA2 Antikörper (AA 278-292) (ABIN1742378)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GRIA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 1404A10

Produktdetails anti-GRIA2 Antikörper

Bindungsspezifität: AA 278-292

Spezifität: Specific for GluA 2.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 278-292 in rat GluA 2, unprocessed precursor) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Kommentare:

IP:For most effective IP use the solubilization protocol described in this ELISA protocol. Consider that protein-protein interaction may be affected.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-GRIA2 Antikörper (ABIN1742378)

Jedlicka, Vnencak, Krueger, Jungenitz, Brose, Schwarzacher: "Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo." in: Brain structure & function, Vol. 220, Issue 1, pp. 47-58, (2015) (PubMed).

Details zu GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Andere Bezeichnung: GluA 2

Hintergrund: Synonyms: AMPA 2, GluR 2

Pathways: PI3K-Akt Signalweg