RAB27A Antikörper

Produktnummer ABIN1742354

Der Kaninchen Polyklonal Anti-RAB27A-Antikörper wurde für WB und IP validiert. Er ist geeignet, RAB27A in Proben von Maus und Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für RAB27A Antikörper (ABIN1742354)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB27A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-RAB27A Antikörper

Spezifität: Specific for rab 27A

Kreuzreaktivität (Details): no cross reaction to rab 27B.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant full length rat rab 27A.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Kommentare:

Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen für anti-RAB27A Antikörper (ABIN1742354)

Hou, Ernst, Stuenkel, Lentz, Williams: "Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125596, (2015) (PubMed).

Lovis, Roggli, Laybutt, Gattesco, Yang, Widmann, Abderrahmani, Regazzi: "Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction." in: Diabetes, Vol. 57, Issue 10, pp. 2728-36, (2008) (PubMed).

Details zu RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Andere Bezeichnung: Rab 27 A

Pathways: Peptide Hormone Metabolism