GFP Antikörper (CypHer5E)

Produktnummer ABIN1742297

Der Kaninchen Polyklonal Anti-GFP-Antikörper wurde für ICC validiert. Er ist geeignet, GFP in Proben von Aequorea victoria zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFP Antikörper (CypHer5E) (ABIN1742297)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit CypHer5E

Applikation: Immunocytochemistry (ICC)

Produktdetails anti-GFP Antikörper (CypHer5E)

Kreuzreaktivität (Details): Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Aufreinigung: Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.

Immunogen: Strep-Tag® fusion protein, full-length GFP.

Anwendungsinformationen

Applikationshinweise: IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Kommentare:

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!

Referenzen für anti-GFP Antikörper (CypHer5E) (ABIN1742297)

Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP