GFP Antikörper (CypHer5E)

Produktnummer ABIN1742297

Der Kaninchen Polyklonal Anti-GFP Antikörper wurde für ICC validiert. Er ist geeignet, GFP in Proben von Aequorea victoria zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFP Antikörper (CypHer5E) (ABIN1742297)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit CypHer5E

Applikation: Immunocytochemistry (ICC)

Produktdetails anti-GFP Antikörper (CypHer5E)

Verwendungszweck: Rabbit anti-GFP antibody

Produktmerkmale: fluorescence-labeled with Cy pH er5E. Cy pH er5E® is a pH -sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. Cy pH er5E® can be detected with the filter sets for Cy5®. Cy pH er5E®: λex 647 nm / λem 667 nm

Aufreinigung: affinity purified

Anwendungsinformationen

Applikationshinweise: WB:N/A IP:N/A ICC:1 : 100 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet

Kommentare:

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS

Konservierungsmittel: Without preservative

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.

Referenzen für anti-GFP Antikörper (CypHer5E) (ABIN1742297)

Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund:

Synonyms: Sirius, meGFP, eGFP, paGFP, acGFP, eYFP, sfGFP, Green fluorescent protein, supereclyptic pH luorin, Cerulean, eCFP, mTFP, Venus, Citrine

Antigen Information: GFP is a widely used green fluorescent protein tag