GFP Antikörper (AA 183-191)

Produktnummer ABIN1742292

Dieses Anti-GFP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GFP in WB. Geeignet für Aequorea victoria. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für GFP Antikörper (AA 183-191) (ABIN1742292)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 101G4

Produktdetails anti-GFP Antikörper

Bindungsspezifität: AA 183-191

Spezifität: Specific for GFP of jelly-fish.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet

Kommentare:

ABIN1742292 is recommended for IP and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-GFP Antikörper (ABIN1742292)

Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, (2013) (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).

Kaddai, Gonzalez, Keslair, Grémeaux, Bonnafous, Gugenheim, Tran, Gual, Le Marchand-Brustel, Cormont: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, (2009) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP