ATF4 Antikörper (AA 25-327) (Atto 488)

Produktnummer ABIN1741055

Der Maus Monoklonal Anti-ATF4-Antikörper wurde für WB, IHC, ICC und IF validiert. Er ist geeignet, ATF4 in Proben von Human zu detektieren.

Kurzübersicht für ATF4 Antikörper (AA 25-327) (Atto 488) (ABIN1741055)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATF4 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: S360A-24

Produktdetails anti-ATF4 Antikörper (Atto 488)

Bindungsspezifität: AA 25-327

Spezifität: Detects ~60 kDa. Does not cross-react with ATF5.

Kreuzreaktivität: Human, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1741055 was sufficient for detection of ATF4 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Andere Bezeichnung: ATF4

Hintergrund: Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.

Gen-ID: 468

NCBI Accession: NP_001666

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response