Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only: WB (2 μg/mL) ICC (10 μg/mL)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS, pH 7.4
Handhabung
Dilute in PBS or medium that is identical to that used in the assay system.
Lagerung
-20 °C
Informationen zur Lagerung
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
Haltbarkeit
12 months
Target
BMF
(Bcl2 Modifying Factor (BMF))
Andere Bezeichnung
Bmf
Hintergrund
In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.