CHEK2 Antikörper (AA 481-531)

Produktnummer ABIN1724714

Der Maus Monoklonal Anti-CHEK2-Antikörper wurde für WB, ELISA, IHC und ICC validiert. Er ist geeignet, CHEK2 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CHEK2 Antikörper (AA 481-531) (ABIN1724714)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: 1C12B8

Produktdetails anti-CHEK2 Antikörper

Bindungsspezifität: AA 481-531

Verwendungszweck: CHK2 Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-CHEK2 Antikörper (ABIN1724714)

Cybulski, Masojc, Oszutowska, Jaworowska, Grodzki, Waloszczyk, Serwatowski, Pankowski, Huzarski, Byrski, Górski, Jakubowska, Debniak, Wokolorczyk, Gronwald, Tarnowska, Serrano-Fernández, Lubinski et al.: "Constitutional CHEK2 mutations are associated with a decreased risk of lung and laryngeal cancers. ..." in: Carcinogenesis, Vol. 29, Issue 4, pp. 762-5, (2008) (PubMed).

Antoni, Sodha, Collins, Garrett: "CHK2 kinase: cancer susceptibility and cancer therapy - two sides of the same coin?" in: Nature reviews. Cancer, Vol. 7, Issue 12, pp. 925-36, (2007) (PubMed).

Bell, Kim, Godwin, Schiripo, Harris, Haserlat, Wahrer, Haiman, Daly, Niendorf, Smith, Sgroi, Garber, Olopade, Le Marchand, Henderson, Altshuler, Haber, Freedman: "Genetic and functional analysis of CHEK2 (CHK2) variants in multiethnic cohorts." in: International journal of cancer. Journal international du cancer, Vol. 121, Issue 12, pp. 2661-7, (2007) (PubMed).

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHK2

Hintergrund: CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 61 kDa

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus