Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only : WB(1 μg/mL)
Positive control: Rat brain lysate.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS, pH 7.4, glycerol 50 % .
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute in PBS or medium that is identical to that used in the assay system.
Lagerung
-20 °C
Informationen zur Lagerung
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
Haltbarkeit
12 months
Target
GIT1
Andere Bezeichnung
GIT1
Hintergrund
G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.
ARF GTPase-activating protein GIT1, ARF GAP GIT1 Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 Gene name : GIT1