Perforin 1 Antikörper (AA 22-100)

Produktnummer ABIN1713880

Produktdetails anti-Perforin 1 Antikörper

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Bindungsspezifität: AA 22-100

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Maus, Ratte

Homologie: Human,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Perforin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin (PRF1 Produkte)

Synonyme: FLH2 antikoerper, HPLH2 antikoerper, P1 antikoerper, PFN1 antikoerper, PFP antikoerper, PRF1 antikoerper, Pfn antikoerper, Pfp antikoerper, Prf-1 antikoerper, Cyta antikoerper, RATCYTA antikoerper, LOC443187 antikoerper, perforin antikoerper, prf1 antikoerper, cytolysin antikoerper, perforin-1 antikoerper, perforin-1-like antikoerper, perforin 1 antikoerper, perforin 1 (pore forming protein) antikoerper, perforin antikoerper, perforin 1 L homeolog antikoerper, PRF1 antikoerper, Prf1 antikoerper, LOC443187 antikoerper, prf1 antikoerper, prf1.L antikoerper

Hintergrund:

Synonyms: Cytolysin, FLH2, HPLH2, Lymphocyte pore forming protein, Lymphocyte pore-forming protein, MGC65093, P1, PERF_HUMAN, Perforin 1, Perforin 1 precursor, Perforin 1 preforming protein, Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF, Pore forming protein, PRF 1, PRF1, SHGC-10760.

Background: The major defense of the body against virus-infected and tumorigenic cells is cytotoxic T lymphocyte (CTL)-mediated cytotoxicity, which also plays a role in autoimmune diseases and transplant rejection. During CTL-mediated cytotoxicity, CTL granules containing perforin are exocytosed. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells by forming transmembrane channels in target cell membranes. Perforin is primarily expressed in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, but has also been observed in an astrocyte population of the human brain. It has been shown that abrogation of perforin function by Ca2+-complexing agents leads to decreased levels of necrosis, demonstrating that both necrosis and apoptosis contribute to CTL-mediated cytotoxicity. Perforin activity has been shown to be induced by IL-2, IL-3, IL-4, IL-6 and to a lesser degree, TNF and IFN-_.

Gen-ID: 5551

Pathways: Apoptose, Caspase Kaskade in der Apoptose