POU2AF1 Antikörper (AA 1-100) (Cy5)

Produktnummer ABIN1704238

Produktdetails anti-POU2AF1 Antikörper (Cy5)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Bindungsspezifität: AA 1-100

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POU2AF1 Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BOB1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Andere Bezeichnung: BOB1 (POU2AF1 Produkte)

Synonyme: BOB1 antikoerper, OBF-1 antikoerper, OBF1 antikoerper, OCAB antikoerper, BOB-1 antikoerper, BOB.1 antikoerper, Bob-1 antikoerper, Bob1 antikoerper, OBF.1 antikoerper, OCA-B antikoerper, POU class 2 associating factor 1 antikoerper, POU domain, class 2, associating factor 1 antikoerper, POU2AF1 antikoerper, Pou2af1 antikoerper

Hintergrund:

Synonyms: B cell Oct binding protein 1, B cell specic coactivator OBF 1, B cell specic coactivator OBF1, B-cell-specic coactivator OBF-1, BOB 1, BOB-1, OBF 1, OBF1, OBF1_HUMAN, OCA B, OCA-B, OCAB, OCT binding factor 1, OCT-binding factor 1, POU domain class 2 associating factor 1, POU domain class 2-associating factor 1, POU2AF1.

Background: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

Gen-ID: 5450