P4HB Antikörper (AA 52-63)

Produktnummer ABIN1686617

Der Maus Monoklonal Anti-P4HB-Antikörper wurde für WB, IHC, ELISA, IP, IF und ICC validiert. Er ist geeignet, P4HB in Proben von Human zu detektieren.

Kurzübersicht für P4HB Antikörper (AA 52-63) (ABIN1686617)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P4HB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 2E7-7

Produktdetails anti-P4HB Antikörper

Bindungsspezifität: AA 52-63

Spezifität: Detects ~48 kDa.

Kreuzreaktivität: Human

Aufreinigung: Protein G Purified

Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:200)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1686617 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI

Hintergrund: Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Gen-ID: 81567

NCBI Accession: NP_001139021

UniProt: Q8NBS9

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism