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RFP Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RFP in WB, ELISA, IP, FACS und FM. Er zeigt eine Reaktivität gegenüber Discosoma und wurde in 16+ Publikationen erwähnt.
Rockland
Produktnummer ABIN1607680
Hersteller Produkt- Nr.: 200-301-379

Kurzübersicht für RFP Antikörper (ABIN1607680)

Target

Alle RFP Antikörper anzeigen
RFP (Red Fluorescent Protein (RFP))

Reaktivität

Discosoma

Wirt

  • 20
  • 14
  • 9
  • 7
  • 3
  • 1
Maus

Klonalität

  • 32
  • 22
Monoklonal

Konjugat

  • 27
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser RFP Antikörper ist unkonjugiert

Applikation

  • 35
  • 23
  • 21
  • 13
  • 12
  • 11
  • 10
  • 10
  • 6
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Klon

8E5-G7
  • Hersteller Produkt- Nr.

    200-301-379

    Hersteller

    Rockland

    Verwendungszweck

    RFP Antibody

    Kreuzreaktivität (Details)

    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

    Produktmerkmale

    Synonyms: mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

    Aufreinigung

    Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

    Immunogen Type: Recombinant Protein

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Application Note: Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:75,000 - 1:150,000

    IF Microscopy Dilution: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Singh, Elenio, Leu, Romano, Vaughan, DeRiso, Surendran, Chakrabarti: "A new Elf5CreERT2-GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues." in: FEBS letters, Vol. 593, Issue 10, pp. 1030-1039, (2020) (PubMed).

    Guan, Quiñones-Frías, Akbergenova, Littleton: "Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner." in: eLife, Vol. 9, (2020) (PubMed).

    Donovan, Spencer, Kitt, Eastman, Lobur, Jiao, Silver, Deneris: "Lmx1b is required at multiple stages to build expansive serotonergic axon architectures." in: eLife, Vol. 8, (2020) (PubMed).

    Winkowski, Nagode, Donaldson, Yin, Shamma, Fritz, Kanold: "Orbitofrontal Cortex Neurons Respond to Sound and Activate Primary Auditory Cortex Neurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 28, Issue 3, pp. 868-879, (2019) (PubMed).

    Katrancha, Shaw, Zhao, Myers, Cocco, Jeng, Zhu, Pittenger, Greer, Carr, Xiao, Koleske: "Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits." in: Cell reports, Vol. 26, Issue 10, pp. 2805-2817.e9, (2019) (PubMed).

    Nicholson, Roberts, Sober: "Thalamostriatal and cerebellothalamic pathways in a songbird, the Bengalese finch." in: The Journal of comparative neurology, Vol. 526, Issue 9, pp. 1550-1570, (2019) (PubMed).

    Bragado Alonso, Reinert, Marichal, Massalini, Berninger, Kuner, Calegari: "An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants." in: The EMBO journal, Vol. 38, Issue 6, (2019) (PubMed).

    Zhou, Rickmann, Hafner, Staiger: "Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 11, pp. 5353-5368, (2018) (PubMed).

    Rider, Cheerathodi, Hurwitz, Nkosi, Howell, Tremblay, Liu, Zhu, Meckes: "The interactome of EBV LMP1 evaluated by proximity-based BioID approach." in: Virology, Vol. 516, pp. 55-70, (2018) (PubMed).

    Guan, Cao, Liu, Zhao, Fu, Yu: "Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex." in: eLife, Vol. 6, (2018) (PubMed).

    Hou, Zhang: "NMDA Receptors Regulate the Development of Neuronal Intrinsic Excitability through Cell-Autonomous Mechanisms." in: Frontiers in cellular neuroscience, Vol. 11, pp. 353, (2017) (PubMed).

    Bifari, Decimo, Pino, Llorens-Bobadilla, Zhao, Lange, Panuccio, Boeckx, Thienpont, Vinckier, Wyns, Bouché, Lambrechts, Giugliano, Dewerchin, Martin-Villalba, Carmeliet: "Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex." in: Cell stem cell, Vol. 20, Issue 3, pp. 360-373.e7, (2017) (PubMed).

    Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

    Clarkson, Liptak, Gee, Sohal, Bender: "D3 Receptors Regulate Excitability in a Unique Class of Prefrontal Pyramidal Cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 24, pp. 5846-5860, (2017) (PubMed).

    Wu, Kim, Seravalli, Barycki, Hart, Gohara, Di Cera, Jung, Kosman, Lee: "Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9796-806, (2016) (PubMed).

    Alexandre, Ghilas, Sanchez, Le Bon, Crozat, Dalod: "XCR1+ dendritic cells promote memory CD8+ T cell recall upon secondary infections with Listeria monocytogenes or certain viruses." in: The Journal of experimental medicine, Vol. 213, Issue 1, pp. 75-92, (2016) (PubMed).

  • Target

    RFP (Red Fluorescent Protein (RFP))

    Andere Bezeichnung

    DsRed

    Hintergrund

    Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.

    UniProt

    Q9U6Y8
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