CREB1 Antikörper (AA 96-109)

Produktnummer ABIN1590024

Dieses Anti-CREB1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von CREB1 in WB, IHC und ELISA. Geeignet für Human und Maus.

Kurzübersicht für CREB1 Antikörper (AA 96-109) (ABIN1590024)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CREB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-CREB1 Antikörper

Bindungsspezifität: AA 96-109

Verwendungszweck: CREB1 (aa96-109)

Sequenz: DSQESVDSVT DSQK

Spezifität: This antibody is expected to recognize both reported isoforms (NP_004370.1, NP_604391.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 45 kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7 kDa according to NP_604391.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primar

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Andere Bezeichnung: CREB1

Hintergrund: CREB1, cAMP responsive element binding protein 1, CREB, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein

Gen-ID: 1385, 12912

NCBI Accession: NP_004370, NP_604391

Pathways: TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation