KIT Antikörper (Internal Region)

Produktnummer ABIN1589992

Dieser Ziege Polyklonal Antikörper detektiert spezifisch KIT in WB, FACS, ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für KIT Antikörper (Internal Region) (ABIN1589992)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-KIT Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: KIT / CD117

Sequenz: KRDSFICSKQ EDH

Spezifität: This antibody is expected to recognize both reported isoforms (NP_000213.1, NP_001087241.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

Western Blot: Approx 110 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110 kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incub

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended co

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: KIT

Hintergrund: KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian

Gen-ID: 3815

NCBI Accession: NP_000213, NP_001087241

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity