KIT Antikörper (Internal Region)

Produktnummer ABIN1589992

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch KIT in WB, FACS, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für KIT Antikörper (Internal Region) (ABIN1589992)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-KIT Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: KIT / CD117

Sequenz: KRDSFICSKQ EDH

Spezifität: This antibody is expected to recognize both reported isoforms (NP_000213.1, NP_001087241.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

Western Blot: Approx 110 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110 kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incub

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended co

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: KIT

Hintergrund: KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian

Gen-ID: 3815

NCBI Accession: NP_000213, NP_001087241

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity