VEGFR2/CD309 Antikörper (Biotin)

Produktnummer ABIN1589929

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch VEGFR2/CD309 in WB. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für VEGFR2/CD309 Antikörper (Biotin) (ABIN1589929)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser VEGFR2/CD309 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB)

Klon: 3C8

Produktdetails anti-VEGFR2/CD309 Antikörper (Biotin)

Verwendungszweck: VEGFR-2/Flk-1 antibody

Spezifität: Recombinant mouse soluble Flk-1

Produktmerkmale: Chromosomal location: 5 C3.3, 5 42.0 cM
Monoclonal antibodies were produced from a murine hybridoma elicited from a rat immunized with purified, insect cell-derived recombinant mouse soluble Flk-1/KDR (110 kDa) as the immunizing antigen.

Aufreinigung: Rat IgG1 antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogen: Recombinant mouse soluble Flk-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: use at 2-5 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Centrifuge vial prior to opening.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Haltbarkeit: 24 months

Details zu VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Andere Bezeichnung: VEGFR-2/Flk-1

Hintergrund: Vascular endothelial growth factor receptor-2 , Kdr, Ly73, Flk-1, VEGF receptor 2, kinase insert domain protein receptor,VEGF R2 (mouseFlk1 gene), VEGF R1 (Flt1) and VEGF R3 (Flt4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulinlike repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The solube receptor was used as a an antigen.

Gen-ID: 16542

UniProt: P35918

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling