VEGFR2/CD309 Antikörper (Biotin)

Produktnummer ABIN1589717

Der Maus Monoklonal Anti-VEGFR2/CD309-Antikörper wurde für WB, IHC, ELISA und FACS validiert. Er ist geeignet, VEGFR2/CD309 in Proben von Human zu detektieren.

Kurzübersicht für VEGFR2/CD309 Antikörper (Biotin) (ABIN1589717)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGFR2/CD309 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 3(4H3

Produktdetails anti-VEGFR2/CD309 Antikörper (Biotin)

Verwendungszweck: VEGFR-2/KDR antibody

Spezifität: Recombinant human soluble extracellular KDR (D7)

Kreuzreaktivität (Details): The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Produktmerkmale: Chromosomal location: 4q11-q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Aufreinigung: Mouse IgG1 antibody (clone 3 (4H3)) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogen: Recombinant human soluble extracellular KDR (D7)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: FACS: Use at 2-10 μg/mL, Western Blot: Use at 2-5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Centrifuge vial prior to opening.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Haltbarkeit: 24 months

Details zu VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Andere Bezeichnung: VEGFR-2/KDR

Hintergrund: Vascular endothelial growth factor receptor-2 , KDR, FLK1, CD309, VEGF receptor 2, VEGFR2, kinase insert domain protein receptor,VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Gen-ID: 3791

NCBI Accession: NM_002253, NP_002244

UniProt: P35968

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling