LIG4 Antikörper (AA 591-640)

Produktnummer ABIN1534440

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LIG4 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für LIG4 Antikörper (AA 591-640) (ABIN1534440)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-LIG4 Antikörper

Bindungsspezifität: AA 591-640

Verwendungszweck: DNA Ligase IV Antibody

Spezifität: DNA Ligase IV Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DNL4. AA range:591-640

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Hintergrund:

Synonyms: LIG4, DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4

Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

Gene Name: LIG4

Protein Name: DNA ligase 4

Molekulargewicht: 103 kDa

Gen-ID: 3981

OMIM: 601837

UniProt: P49917

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response