FEN1 Antikörper (AA 86-135)

Produktnummer ABIN1533720

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch FEN1 in WB, IHC, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für FEN1 Antikörper (AA 86-135) (ABIN1533720)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-FEN1 Antikörper

Bindungsspezifität: AA 86-135

Verwendungszweck: FEN-1 Antibody

Spezifität: FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FEN1. AA range:86-135

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: FEN1

Hintergrund:

Synonyms: FEN1; RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage byThe protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions

Gene Name: FEN1

Protein Name: Flap endonuclease 1

Molekulargewicht: 42 kDa

Gen-ID: 2237

OMIM: 600393

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA