PDPK1 Antikörper (pSer241)

Produktnummer ABIN1531949

Der Kaninchen Polyklonal anti-PDPK1 Antikörper wird verwendet zum Nachweis von PDPK1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für PDPK1 Antikörper (pSer241) (ABIN1531949)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-PDPK1 Antikörper

Bindungsspezifität: AA 210-259, pSer241

Verwendungszweck: PDPK1 (phospho Ser241) Antibody

Spezifität: Phospho-PDK1 (S241) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at S241.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Ser241. AA range:210-259

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung: PDK1

Hintergrund:

Synonyms: PDPK1, PDK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,

Gene Name: PDPK1

Protein Name: 3-phosphoinositide-dependent protein kinase 1

Molekulargewicht: 63 kDa

Gen-ID: 5170

OMIM: 605213

UniProt: O15530

Pathways: PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals