CHEK2 Antikörper (pThr383)

Produktnummer ABIN1531498

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CHEK2 in WB, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus und Affe.

Kurzübersicht für CHEK2 Antikörper (pThr383) (ABIN1531498)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-CHEK2 Antikörper

Bindungsspezifität: AA 356-405, pThr383

Verwendungszweck: Chk2 (phospho Thr383) Antibody

Spezifität: Phospho-Chk2 (T383) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T383.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr383. AA range:356-405

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: Chk2

Hintergrund:

Synonyms: CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators.The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati

Gene Name: CHEK2

Protein Name: Serine/threonine-protein kinase Chk2

Molekulargewicht: 60 kDa

Gen-ID: 11200

OMIM: 176807

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus