Cyclin E1 Antikörper (pThr77)

Produktnummer ABIN1531430

Der Kaninchen Polyklonal anti-Cyclin E1 Antikörper wird verwendet zum Nachweis von Cyclin E1 in Proben von Human, Ratte und Maus. Er wurde validiert für IHC, ELISA und IF.

Kurzübersicht für Cyclin E1 Antikörper (pThr77) (ABIN1531430)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: AA 43-92, pThr77

Verwendungszweck: Cyclin E1 (phospho Thr77) Antibody

Spezifität: Phospho-Cyclin E1 (T77) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T77.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77. AA range:43-92

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E1

Hintergrund:

Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

Gene Name: CCNE1

Protein Name: G1/S-specific cyclin-E1

Molekulargewicht: 47 kDa

Gen-ID: 898

OMIM: 123837

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases