PKC zeta Antikörper (pThr410)

Produktnummer ABIN1531192

Der Kaninchen Polyklonal anti-PKC zeta Antikörper wird verwendet zum Nachweis von PKC zeta in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für PKC zeta Antikörper (pThr410) (ABIN1531192)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC zeta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-PKC zeta Antikörper

Bindungsspezifität: AA 376-425, pThr410

Verwendungszweck: PKC ζ (phospho Thr410) Antibody

Spezifität: Phospho-PKC ζ (T410) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T410.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr410. AA range:376-425

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Andere Bezeichnung: PKC zeta

Hintergrund:

Synonyms: PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zeta

Background: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Gene Name: PRKCZ

Protein Name: Protein kinase C zeta type

Molekulargewicht: 67 kDa

Gen-ID: 5590

OMIM: 176982

UniProt: Q05513

Pathways: NF-kappaB Signalweg, RTK Signalweg, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling