KIT Ligand Antikörper

Produktnummer ABIN1500815

Der Ziege Polyklonal Anti-KIT Ligand Antikörper wurde für WB, IHC, ELISA, IHC (p) und Neut validiert. Er ist geeignet, KIT Ligand in Proben von Human zu detektieren.

Kurzübersicht für KIT Ligand Antikörper (ABIN1500815)

Target: KIT Ligand (KITLG)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Produktdetails anti-KIT Ligand Antikörper

Marke: IHC-plus™

Spezifität: Human SCF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human SCF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.01 - 0.01 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8209 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (100 ng/mL), a concentration of 008-012 μg/mL of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104803) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: KITLG / SCF

Hintergrund: Name/Gene ID: KITLG

Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

Gen-ID: 4254

UniProt: P21583

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung