ATG4A Antikörper (C-Term)

Produktnummer ABIN1449633

Dieses Anti-ATG4A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG4A in WB, IHC (p) und EIA. Geeignet für Human und Maus.

Kurzübersicht für ATG4A Antikörper (C-Term) (ABIN1449633)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ATG4A Antikörper

Bindungsspezifität: AA 369-398, C-Term

Aufreinigung: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 369~398 amino acids from the C-term of Human APG4A

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Andere Bezeichnung: Autophagin-2

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.Synonyms: APG4A, ATG4A, AUT-like 2 cysteine endopeptidase, AUTL2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, Cysteine protease ATG4A

Molekulargewicht: 45378 Da

Gen-ID: 115201

NCBI Accession: NP_443168

Pathways: Autophagie