LIN28A Antikörper (AA 65-160) (Cy5)

Produktnummer ABIN1415219

Der Kaninchen Polyklonal Anti-LIN28A Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, LIN28A in Proben von Human und Ratte zu detektieren.

Kurzübersicht für LIN28A Antikörper (AA 65-160) (Cy5) (ABIN1415219)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN28A Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-LIN28A Antikörper (Cy5)

Bindungsspezifität: AA 65-160

Kreuzreaktivität: Human, Ratte

Homologie: Mouse,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lin-28B

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Andere Bezeichnung: Lin-28

Hintergrund:

Synonyms: Lin28B, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B C. elegans, Lin 28.2, Lin-28B, LIN28B, LN28B_HUMAN, Protein lin-28 homolog B, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Gen-ID: 389421, 79727

UniProt: Q6ZN17, Q9H9Z2

Pathways: Stem Cell Maintenance