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LIN28A Antikörper (N-Term)

LIN28A Reaktivität: Human, Rind (Kuh), Hund, Schwein, Schaf WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6741558
  • Target Alle LIN28A Antikörper anzeigen
    LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
    Bindungsspezifität
    • 15
    • 9
    • 9
    • 7
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 102
    • 29
    • 21
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rind (Kuh), Hund, Schwein, Schaf
    Wirt
    • 81
    • 23
    Kaninchen
    Klonalität
    • 74
    • 30
    Polyklonal
    Konjugat
    • 49
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser LIN28A Antikörper ist unkonjugiert
    Applikation
    • 68
    • 41
    • 30
    • 21
    • 16
    • 14
    • 13
    • 13
    • 10
    • 9
    • 7
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human LIN28A / LIN28
    Homologie
    Percent identity by BLAST analysis: Dog, Bovine, Pig (100%) Rat, Sheep (85%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide from N-Terminus of human LIN28A (Q9H9Z2, NP_078950). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Dog, Cat, Bovine, Pig (100%), Rabbit (92%), Monkey, Mouse, Rat, Elephant (90%), Sheep, Guinea pig (85%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
    Top Product
    Discover our top product LIN28A Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
    Andere Bezeichnung
    LIN28A / LIN28 (LIN28A Produkte)
    Synonyme
    CSDD1 antikoerper, LIN-28 antikoerper, LIN28 antikoerper, ZCCHC1 antikoerper, lin-28A antikoerper, AL024421 antikoerper, ENSMUSG00000070700 antikoerper, Gm10299 antikoerper, Lin-28 antikoerper, Lin28 antikoerper, Tex17 antikoerper, csdd1 antikoerper, lin-28 antikoerper, lin28 antikoerper, zcchc1 antikoerper, sb:cb650 antikoerper, zgc:55584 antikoerper, RGD1566408 antikoerper, lin-28 homolog A antikoerper, lin-28 homolog A (C. elegans) antikoerper, lin-28 homolog A S homeolog antikoerper, LIN28A antikoerper, Lin28a antikoerper, lin28a.S antikoerper, lin28a antikoerper
    Hintergrund
    Name/Gene ID: LIN28A

    Synonyms: LIN28A, CSDD1, Lin-28 homolog (C. elegans), LIN-28, Lin-28A, LIN28, Protein lin-28 homolog A, Lin-28 homolog A (C. elegans), ZCCHC1, RNA-binding protein LIN-28
    Gen-ID
    79727
    NCBI Accession
    NP_078950
    UniProt
    Q9H9Z2
    Pathways
    Stem Cell Maintenance
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