MAPK12 Antikörper (pThr183, pTyr185)

Produktnummer ABIN1387757

Der Kaninchen Polyklonal Anti-MAPK12-Antikörper wurde für WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc) und IF (p) validiert. Er ist geeignet, MAPK12 in Proben von Human zu detektieren.

Kurzübersicht für MAPK12 Antikörper (pThr183, pTyr185) (ABIN1387757)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPK12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-MAPK12 Antikörper

Bindungsspezifität: pThr183, pTyr185

Homologie: Human,Mouse,Rat,Dog,Cow,Pig,Chicken,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SAPK3 around the phosphorylation site of Thr183+Tyr185 [EM(p-T)G(p-Y)VV]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Andere Bezeichnung: SAPK3+

Hintergrund:

Synonyms: p-SAPK3Thr183+Tyr185, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, Mapk12, Mitogen Activated Protein Kinase 12, ERK6, ERK 6, ERK-6, Extracellular signal-regulated kinase 6, Mitogen activated protein kinase 3, Mitogen activated protein kinase p38 gamma, Mitogen-activated protein kinase 12, Mitogen-activated protein kinase p38 gamma, MK12_HUMAN, P38 GAMMA, P38GAMMA, PRKM12, SAPK 3, SAPK3, Stress Activated Protein Kinase 3, Stress-activated protein kinase 3.

Background: MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38 alpha, p38 beta and p38 gamma, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38 alpha, p38 beta and p38 gamma are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteine