NLRP3 Antikörper (AA 15-120)

Produktnummer ABIN1386361

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NLRP3 in WB, ELISA, IHC (p), FACS, ICC, IHC (fro), IF (p) und IF (cc). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund und wurde in 11+ Publikationen erwähnt.

Kurzübersicht für NLRP3 Antikörper (AA 15-120) (ABIN1386361)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-NLRP3 Antikörper

Bindungsspezifität: AA 15-120

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NLRP3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-NLRP3 Antikörper (ABIN1386361)

Li, Jiang, Ye, Zhou, Liu, Yang, Hao, Hu: "P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages." in: Cell death & disease, Vol. 11, Issue 5, pp. 394, (2021) (PubMed).

Yang, Cheng, Liu, Liu, Tan, Guan, Guo, Kuang: "Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway." in: Molecules (Basel, Switzerland), Vol. 24, Issue 11, (2019) (PubMed).

Zendedel, Mönnink, Hassanzadeh, Zaminy, Ansar, Habib, Slowik, Kipp, Beyer: "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." in: Molecular neurobiology, (2017) (PubMed).

Yu, Liu, Sun, Wang, Wang, Zhao, Dai, Zhao, Zhang, Li, Han, Lu, Dong, Liu, Yu, Li: "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." in: Oncotarget, Vol. 8, Issue 23, pp. 37705-37716, (2017) (PubMed).

Zhang, Pan, Yang, Fu, Liu, Chi, Zhang, Hong, Ma, Yin: "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 43, Issue 4, pp. 1311-1324, (2017) (PubMed).

Fernandez, Miller, Krammer, Gopal, Greenbaum, Bhardwaj: "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." in: Journal of leukocyte biology, Vol. 99, Issue 5, pp. 723-34, (2016) (PubMed).

Zhou, Rivera, Bhosle, Lahaie, Shao, Tahiri, Zhu, Polosa, Dorfman, Beaudry-Richard, Costantino, Lodygensky, Lachapelle, Chemtob: "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." in: The American journal of pathology, Vol. 186, Issue 12, pp. 3100-3116, (2016) (PubMed).

Debye, Schmülling, Zhou, Rune, Beyer, Johann: "Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice." in: Brain pathology (Zurich, Switzerland), (2016) (PubMed).

Heitzer, Kaiser, Kanagaratnam, Zendedel, Hartmann, Beyer, Johann: "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1(G93A) ALS Mice." in: Molecular neurobiology, (2016) (PubMed).

Zendedel, Johann, Mehrabi, Joghataei, Hassanzadeh, Kipp, Beyer: "Activation and Regulation of NLRP3 Inflammasome by Intrathecal Application of SDF-1a in a Spinal Cord Injury Model." in: Molecular neurobiology, (2015) (PubMed).

Johann, Heitzer, Kanagaratnam, Goswami, Rizo, Weis, Troost, Beyer: "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." in: Glia, (2015) (PubMed).

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: CIAS1

Hintergrund:

Synonyms: NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3, C1orf7, CIAS1, NALP3, PYPAF1

Background: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

Gen-ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome