NLRP3 Antikörper (AA 21-194)

Produktnummer ABIN3043571

Produktdetails anti-NLRP3 Antikörper

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Bindungsspezifität: AA 21-194

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3(NLRP3) detection. Tested with WB in Human.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3(NLRP3) detection. Tested with WB in Human.
Gene Name: NLR family, pyrin domain containing 3
Protein Name: NACHT, LRR and PYD domains-containing protein 3

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CIAS1 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-NLRP3 Antikörper (ABIN3043571)

Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Xue, Wang, Du, Zhu: "Maternal obesity induces gut inflammation and impairs gut epithelial barrier function in nonobese diabetic mice." in: The Journal of nutritional biochemistry, Vol. 25, Issue 7, pp. 758-64, (2014) (PubMed).

Wang, Yang, Liang, Zhu, Du: "Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice." in: BMC complementary and alternative medicine, Vol. 14, pp. 162, (2014) (PubMed).

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3 (NLRP3 Produkte)

Synonyme: CIAS1 antikoerper, Nalp3 antikoerper, avp antikoerper, cias1 antikoerper, nalp3 antikoerper, pypaf1 antikoerper, agtavprl antikoerper, AGTAVPRL antikoerper, AII antikoerper, AVP antikoerper, C1orf7 antikoerper, CLR1.1 antikoerper, FCAS antikoerper, FCU antikoerper, MWS antikoerper, NALP3 antikoerper, PYPAF1 antikoerper, AII/AVP antikoerper, Cias1 antikoerper, Mmig1 antikoerper, Pypaf1 antikoerper, NLR family pyrin domain containing-like antikoerper, NLR family pyrin domain containing 3 antikoerper, NLR family, pyrin domain containing 3 L homeolog antikoerper, NLR family, pyrin domain containing 3 antikoerper, NLRPL antikoerper, NLRP3 antikoerper, nlrp3.L antikoerper, Nlrp3 antikoerper

Hintergrund: NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Synonyms: AGTAVPRL antibody|AII/AVP antibody|Angiotensin/vasopressin receptor AII/AVP like antibody|Angiotensin/vasopressin receptor AII/AVP-like antibody|C1orf7 antibody|Caterpiller protein 1.1 antibody|CIAS 1 antibody|CIAS1 antibody|CLR1.1 antibody|Cold autoinflammatory syndrome 1 antibody|Cold autoinflammatory syndrome 1 protein antibody|Cryopyrin antibody|Familial cold autoinflammatory syndrome antibody|FCAS antibody|FCU antibody|LRR and PYD domains-containing protein 3 antibody|Muckle-Wells syndrome antibody|MWS antibody|NACHT antibody|NACHT LRR and PYD containing protein 3 antibody|NALP 3 antibody|NALP3 antibody|NALP3_HUMAN antibody|NLRP3 antibody|PYPAF 1 antibody|PYPAF1 antibody|PYRIN containing APAF1 like protein 1 antibody|PYRIN-containing APAF1-like protein 1 antibody

Gen-ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome