Influenza Nucleoprotein Antikörper (Influenza A Virus (A/Russia:St.Petersburg/8/2006)) (AA 71-170)

Produktnummer ABIN1385576

Produktdetails

Target: Influenza Nucleoprotein (NP)

Bindungsspezifität: AA 71-170

Reaktivität: Influenza A Virus, Virus

Virus Strain: A/Russia:St.Petersburg/8/2006

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): Influenza A virus

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St.Petersburg/8/2006 H1N1) Nucleoprotein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen

Chen, Angel, Li, Finch, Gonzalez, Sutton, Santos, Perez: "All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines." in: Journal of virology, Vol. 88, Issue 17, pp. 10013-25, (2014) (PubMed).

Antigendetails

Target: Influenza Nucleoprotein (NP)

Andere Bezeichnung: Influenza A virus Nucleoprotein (NP Produkte)

Substanzklasse: Influenza Protein

Hintergrund:

Synonyms: Nucleoprotein, NP, Nucleocapsid protein, Protein N, Influenza A virus H1N1, H3N2 Nucleoprotein, H9N2 Nucleoprotein, H2N2 Nucleoprotein, H3N8 Nucleoprotein, H7N7 Nucleoprotein, H5N1 Nucleoprotein.

Background: Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.