CD44 Antikörper

Produktnummer ABIN135065

Der Ratte Monoklonal Anti-CD44-Antikörper wurde für FACS validiert. Er ist geeignet, CD44 in Proben von Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CD44 Antikörper (ABIN135065)

Target: CD44

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: KM201

Produktdetails anti-CD44 Antikörper

Spezifität: Mouse CD44 (all isoforms), Mr 80-95 kDa

Produktmerkmale: Rat Anti-Mouse CD44-UNLB

Aufreinigung: Purified

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Kommentare:

cell adhesion and homing

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Konservierungsmittel: Without preservative

Handhabung: Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen für anti-CD44 Antikörper (ABIN135065)

Rux, Song, Swinehart, Pineault, Schlientz, Trulik, Goldstein, Kozloff, Lucas, Wellik: "Regionally Restricted Hox Function in Adult Bone Marrow Multipotent Mesenchymal Stem/Stromal Cells." in: Developmental cell, Vol. 39, Issue 6, pp. 653-666, (2017) (PubMed).

Goldman, Majumder, Dhawan, Ravi, Goldman, Kohandel, Majumder, Sengupta: "Temporally sequenced anticancer drugs overcome adaptive resistance by targeting a vulnerable chemotherapy-induced phenotypic transition." in: Nature communications, Vol. 6, pp. 6139, (2016) (PubMed).

Sánchez, Hill, Love, Soslow, Craig, Austin, Brown, Czirok, Camenisch, Barnett: "The cytoplasmic domain of TGFβR3 through its interaction with the scaffolding protein, GIPC, directs epicardial cell behavior." in: Developmental biology, Vol. 358, Issue 2, pp. 331-43, (2011) (PubMed).

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Hintergrund: CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling