CD3 Antikörper

Produktnummer ABIN1302512

Der Hamster Monoklonal Anti-CD3-Antikörper wurde für FACS, IP, IHC (fro), Func und ICC validiert. Er ist geeignet, CD3 in Proben von Maus zu detektieren. Es sind 9+ Publikationen verfügbar.

Kurzübersicht für CD3 Antikörper (ABIN1302512)

Target: CD3

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunocytochemistry (ICC)

Klon: 145-2C11

Produktdetails anti-CD3 Antikörper

Verwendungszweck: Anti-Ms CD3 Purified Low Endotoxin

Spezifität: The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.

Kreuzreaktivität (Details): Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Mouse BM10-37 cytotoxic T lymphocytes

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Functional application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion.
Flow cytometry: Recommended dilution: 1-2 μg / ml (million cells).
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg protein in 1 mL of a cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD3 Antikörper (ABIN1302512)

Han, Murray-Segal, Gershenzon, Zhang, Hodder, Pietersz, Mottram: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, (2000) (PubMed).

Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, (1998) (PubMed).

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, (1995) (PubMed).

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, (1995) (PubMed).

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, (1994) (PubMed).

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, (1994) (PubMed).

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, (1991) (PubMed).

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

Samelson, OShea, Luong, Ross, Urdahl, Klausner, Bluestone: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, (1987) (PubMed).

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

Gen-ID: 12501

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway