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Nitrotyrosine Antikörper

Reaktivität: Diverse Spezies WB, IHC (fro), IHC (p) Wirt: Maus Monoclonal EM-30 unconjugated
Produktnummer ABIN1302502
  • Target Alle Nitrotyrosine Antikörper anzeigen
    Nitrotyrosine
    Reaktivität
    • 20
    • 16
    • 16
    • 1
    Diverse Spezies
    Wirt
    • 25
    • 23
    • 5
    Maus
    Klonalität
    • 28
    • 25
    Monoklonal
    Konjugat
    • 21
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Nitrotyrosine Antikörper ist unkonjugiert
    Applikation
    • 51
    • 27
    • 25
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    • 14
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Spezifität
    The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
    Kreuzreaktivität (Details)
    Broad
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    NO2-Tyr-CH2-Thyroglobulin
    Klon
    EM-30
    Isotyp
    IgG1
    Top Product
    Discover our top product Nitrotyrosine Primärantikörper
  • Applikationshinweise
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Western blotting: positive control: NO2-BSA, NO2-HSA.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Target
    Nitrotyrosine
    Abstract
    Nitrotyrosine Produkte
    Substanzklasse
    Chemical
    Hintergrund
    Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
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