Nitrotyrosine Antikörper
Kurzübersicht für Nitrotyrosine Antikörper (ABIN1302502)
Target
Alle Nitrotyrosine Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Anti-Nitrotyrosine Purified
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Spezifität
- The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
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Kreuzreaktivität (Details)
- Broad
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Aufreinigung
- Purified by protein-A affinity chromatography.
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Reinheit
- > 95 % (by SDS-PAGE)
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Immunogen
- NO2-Tyr-CH2-Thyroglobulin
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Isotyp
- IgG1
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Applikationshinweise
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Do not freeze.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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- Nitrotyrosine
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Substanzklasse
- Chemical
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Hintergrund
- Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
Target
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