Nitrotyrosine Antikörper

Produktnummer ABIN1302502

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nitrotyrosine in WB, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Diverse Spezies.

Kurzübersicht für Nitrotyrosine Antikörper (ABIN1302502)

Target: Nitrotyrosine

Reaktivität: Diverse Spezies

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: EM-30

Produktdetails anti-Nitrotyrosine Antikörper

Verwendungszweck: Anti-Nitrotyrosine Purified

Spezifität: The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.

Kreuzreaktivität (Details): Broad

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: NO2-Tyr-CH2-Thyroglobulin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical

Hintergrund: Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO₂, C₉H₁₀N₂O₅