SFRP1 Antikörper

Produktnummer ABIN129592

Hersteller Produkt- Nr.: 600-401-475

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SFRP1 in WB, IHC, ELISA und FM. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für SFRP1 Antikörper (ABIN129592)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SFRP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-SFRP1 Antikörper

Hersteller Produkt- Nr.: 600-401-475

Hersteller: Rockland

Verwendungszweck: SFRP1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human SFRP protein.

Produktmerkmale: Synonyms: rabbit anti-SFRP1 antibody, SFRP-1, secreted frizzled related protein 1 antibody, FRP-1 antibody, FRP 1 antibody, FRP1 antibody, FrzA antibody, SARP 2 antibody, SARP2 antibody, Secreted Apoptosis-related Protein 2 antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:800

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and IHC.  This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200-1:2,000

ELISA Dilution: 1:5,000 - 1:15,000

IF Microscopy Dilution: Use at a concentration of 5 μg/mL

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SFRP1 Antikörper (ABIN129592)

Huang, Zhang, Teng, Lin, Zheng, Yang, Han: "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma." in: BMC cancer, Vol. 7, pp. 126, (2007) (PubMed).

Garcia-Hoyos, Cantalapiedra, Arroyo, Esteve, Rodríguez, Riveiro, Trujillo, Ramos, Bovolenta, Ayuso: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." in: Molecular vision, Vol. 10, pp. 426-31, (2004) (PubMed).

Han, Amar: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, (2004) (PubMed).

Details zu SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Andere Bezeichnung: SFRP1

Hintergrund: Background: Anti-SFRP1 is a Stem Cell Antibody. SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Gen-ID: 6422, 56117838

UniProt: Q8N474

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation