Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Concentration Definition: by UV absorbance at 280 nm
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.
This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).
PLK, STPK13, plk, PLK-1, Plx1, stpk13, Plk, cb525, cb636, ik:tdsubc_2d1, wu:fb37g11, wu:fb76g03, xx:tdsubc_2d1, polo like kinase 1, polo like kinase 1 S homeolog, polo-like kinase 1, polo-like kinase 1 (Drosophila), Serine/threonine-protein kinase plk-1, PLK1, plk1.S, plk1, Plk1, plk-1
Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains. Synonyms: PLK 1 antibody, PLK antibody, polio like kinase antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody