CENPE Antikörper

Produktnummer ABIN129515

Hersteller Produkt- Nr.: 200-301-901

Der Maus Monoklonal Anti-CENPE-Antikörper wurde für WB, ELISA, IP, IF und FM validiert. Er ist geeignet, CENPE in Proben von Human zu detektieren.

Kurzübersicht für CENPE Antikörper (ABIN129515)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CENPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: 1H12

Produktdetails anti-CENPE Antikörper

Hersteller Produkt- Nr.: 200-301-901

Hersteller: Rockland

Verwendungszweck: CENP-E

Kreuzreaktivität (Details): This Protein A purified antibody is directed against human CENP-E protein.

Produktmerkmale: Synonyms: mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE

Aufreinigung: The product was purified from tissue culture supernatant by chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Andere Bezeichnung: CENPE

Hintergrund: Background: CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G₂ phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase.  CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Gen-ID: 1062

UniProt: Q02224

Pathways: M Phase, Maintenance of Protein Location