PARP1 Antikörper (Asp214, C-Term, cleaved)

Produktnummer ABIN127025

Der Kaninchen Polyklonal anti-PARP1 Antikörper wird verwendet zum Nachweis von PARP1 in Proben von Maus. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für PARP1 Antikörper (Asp214, C-Term, cleaved) (ABIN127025)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PARP1 Antikörper

Bindungsspezifität: Asp214, C-Term, cleaved

Spezifität: The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to the C-terminus adjacent to Asp214 of mouse PARP

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1

Hintergrund: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavege occurs between ASP214 and Gly 215, which separates PARP's N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1

Gen-ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location