Calreticulin Antikörper (C-Term)

Produktnummer ABIN126998

Der Kaninchen Polyklonal Anti-Calreticulin-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, Calreticulin in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für Calreticulin Antikörper (C-Term) (ABIN126998)

Target: Calreticulin (CALR)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calreticulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Calreticulin Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes a 60 kDa calreticulin on SDS-PAGE immunoblots.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide from the C-terminus of Human Calreticulin

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-2 μg/mL. Immunohistochemistry. NIH/3T3 cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS containing 1 mg/mL BSA, 1.5 mM sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Details zu Calreticulin

Target: Calreticulin (CALR)

Andere Bezeichnung: Calreticulin

Hintergrund: Calreticulin is a calcium binding protein found in abundance in the endoplasmic reticulum and the sarcoplasmic reticulum. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus. Calreticulin has also been detected in the nucleus and nuclear envelop. Recent studies suggest that this soluble ER protein has a multifunctional role as it appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. Studies also suggest its involvement in certain autoimmune diseases. Consistence with its multiple functions the calreticulin molecule appears to have a zonal character. The protein has both high and low affinity calcium binding sites.Synonyms: CALR, CRP55, CRTC, Calregulin, ERp60, HACBP, grp60

Gen-ID: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response